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prolong gold antifade medium p36930  (Thermo Fisher)


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    Thermo Fisher prolong gold antifade medium p36930
    Prolong Gold Antifade Medium P36930, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antifade+medium+prolong+gold/prolong+diamond+antifade+mountant/bio_rxiv__2025__07__16__665215-247-17-22
    Average 90 stars, based on 1 article reviews
    prolong gold antifade medium p36930 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Schwann cell–derived periostin promotes autoimmune peripheral polyneuropathy via macrophage recruitment
    Article Snippet: Sections were washed with PBS and incubated with 1:200 Alexa Fluor 594–labeled goat polyclonal anti-rabbit secondary antibody (Invitrogen, Thermo Fisher Scientific, catalog A11037) and 1:200 Alexa Fluor 488–labeled goat polyclonal antimouse secondary antibody (Invitrogen, catalog A11001) at room temperature for 1 hour. .. After incubation with a secondary antibody, slides were washed with PBS and coverslipped using antifade medium containing 4′,6-diamidino-2-phenylindole (Prolong Gold, Invitrogen, Thermo Fisher Scientific, catalog P36931) and photographed using a Zeiss 800 confocal microscope. ..

    Article Title: Dynamic seizure-related changes in extracellular signal-regulated kinase activation in a mouse model of temporal lobe epilepsy
    Article Snippet: .. After thorough rinsing, sections were incubated in a mixture of goat anti-rabbit IgG conjugated to Alexa Fluor 488 and goat anti-mouse IgG conjugated to Alexa Fluor 555 (both 1:500; Invitrogen) at RT for 4 h. After rinsing, sections were mounted on slides and coverslipped with antifade medium Prolong Gold (Invitrogen). .. Sections with single labeling for pERK, ERK, NeuN and Fos were analyzed, and digital images were obtained with a Zeiss (Thornwood, NY) Axioskop 2 microscope equipped with an AxioCam digital camera system and AxioVision 4.6 software.

    Article Title: Activation of ERK by spontaneous seizures in neural progenitors of the dentate gyrus in a mouse model of epilepsy
    Article Snippet: The secondary antibodies were labeled with either Alexa Fluor (Invitrogen, Eugene, OR) or Dylight (Jackson ImmunoResearch, West Grove, PA), and all secondary antibodies were at a concentration of 1:500 (for further details see ). .. After the incubations, all sections were rinsed in TBS for 30 min, mounted on slides, and coverslipped with the antifade medium Prolong Gold (Invitrogen, Carlsbad, CA). table ft1 table-wrap mode=article t1 caption a4 Antibodies used in Double Labeling and Triple Labeling For immunofluorescence labeling with mouse antibodies to pERK, Ki67 and Nestin or rabbit Pax6 antiserum, sections were processed with water bath antigen-retrieval methods to improve labeling before incubation in primary antibodies ( Peng et al., 2002 ). ..

    Microscopy:

    Article Title: Schwann cell–derived periostin promotes autoimmune peripheral polyneuropathy via macrophage recruitment
    Article Snippet: Sections were washed with PBS and incubated with 1:200 Alexa Fluor 594–labeled goat polyclonal anti-rabbit secondary antibody (Invitrogen, Thermo Fisher Scientific, catalog A11037) and 1:200 Alexa Fluor 488–labeled goat polyclonal antimouse secondary antibody (Invitrogen, catalog A11001) at room temperature for 1 hour. .. After incubation with a secondary antibody, slides were washed with PBS and coverslipped using antifade medium containing 4′,6-diamidino-2-phenylindole (Prolong Gold, Invitrogen, Thermo Fisher Scientific, catalog P36931) and photographed using a Zeiss 800 confocal microscope. ..

    Labeling:

    Article Title: Activation of ERK by spontaneous seizures in neural progenitors of the dentate gyrus in a mouse model of epilepsy
    Article Snippet: The secondary antibodies were labeled with either Alexa Fluor (Invitrogen, Eugene, OR) or Dylight (Jackson ImmunoResearch, West Grove, PA), and all secondary antibodies were at a concentration of 1:500 (for further details see ). .. After the incubations, all sections were rinsed in TBS for 30 min, mounted on slides, and coverslipped with the antifade medium Prolong Gold (Invitrogen, Carlsbad, CA). table ft1 table-wrap mode=article t1 caption a4 Antibodies used in Double Labeling and Triple Labeling For immunofluorescence labeling with mouse antibodies to pERK, Ki67 and Nestin or rabbit Pax6 antiserum, sections were processed with water bath antigen-retrieval methods to improve labeling before incubation in primary antibodies ( Peng et al., 2002 ). ..

    Immunofluorescence:

    Article Title: Activation of ERK by spontaneous seizures in neural progenitors of the dentate gyrus in a mouse model of epilepsy
    Article Snippet: The secondary antibodies were labeled with either Alexa Fluor (Invitrogen, Eugene, OR) or Dylight (Jackson ImmunoResearch, West Grove, PA), and all secondary antibodies were at a concentration of 1:500 (for further details see ). .. After the incubations, all sections were rinsed in TBS for 30 min, mounted on slides, and coverslipped with the antifade medium Prolong Gold (Invitrogen, Carlsbad, CA). table ft1 table-wrap mode=article t1 caption a4 Antibodies used in Double Labeling and Triple Labeling For immunofluorescence labeling with mouse antibodies to pERK, Ki67 and Nestin or rabbit Pax6 antiserum, sections were processed with water bath antigen-retrieval methods to improve labeling before incubation in primary antibodies ( Peng et al., 2002 ). ..



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    a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), <t>DAPI</t> + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. a and d, Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. Quantified data in b,c,e,k,l are represented as mean ± s.e.m., unpaired two-sided t-test. For all experiments, PS19/E4, n = 35; and PS19/E3 n = 42. Pearson’s correlation analysis (two-sided) used for f-i .
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    Image Search Results


    a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. a and d, Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. Quantified data in b,c,e,k,l are represented as mean ± s.e.m., unpaired two-sided t-test. For all experiments, PS19/E4, n = 35; and PS19/E3 n = 42. Pearson’s correlation analysis (two-sided) used for f-i .

    Journal: bioRxiv

    Article Title: Neuronal APOE4 drives damaging lipid accumulation via contact-dependent neuron-oligodendrocyte-microglia interaction in Alzheimer’s disease

    doi: 10.64898/2025.12.04.692390

    Figure Lengend Snippet: a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. a and d, Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. Quantified data in b,c,e,k,l are represented as mean ± s.e.m., unpaired two-sided t-test. For all experiments, PS19/E4, n = 35; and PS19/E3 n = 42. Pearson’s correlation analysis (two-sided) used for f-i .

    Article Snippet: Coverslips were mounted to microscope slides with VECTASHIELD Prolong Gold with DAPI (H-1200-10, Vectors Labs) or without DAPI if the 405 channel was occupies (P36930, Vector Labs).

    Techniques:

    a, Representative immunofluorescent images of BODIPY + neutral lipids and GFAP + astrocyte in the hippocampal DG of PS19/E4 and PS19/E3 mice at 10 months of age. b-e, Correlations between BODIPY + neutral lipids in DG (% area) and Iba1 + % area ( n = 42) ( b ), AT8 + % area ( n = 31) ( c ), NeuN + % area ( n = 39) ( d ), and hippocampal volume ( n = 39) ( e ) in PS19/E3 mice, assessed via Pearson’s correlation analysis (two-sided). f, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of PS19/E4 mice at 4.0, 6.2, 7.8, and 10.0 months of age. Scale bars, 25μm. g, Correlation between age (in months) and BODIPY + neutral lipids in the hippocampal DG of PS19/E4 mice ( n = 20). Nonlinear regression R 2 . h-j, Representative immunofluorescent images of Rab5 + early endosomes (red, h ), Rab7 + late endosomes (red, i ), and LAMP1 + lysosomes (red, j ) with Iba1 + microglia (blue) and BODIPY + neutral lipid (green) in PS19/E4 mice. All scale bars, 40μm. k, Quantification of BODIPY + marker inside LAMP1 + stain ( n = 15).

    Journal: bioRxiv

    Article Title: Neuronal APOE4 drives damaging lipid accumulation via contact-dependent neuron-oligodendrocyte-microglia interaction in Alzheimer’s disease

    doi: 10.64898/2025.12.04.692390

    Figure Lengend Snippet: a, Representative immunofluorescent images of BODIPY + neutral lipids and GFAP + astrocyte in the hippocampal DG of PS19/E4 and PS19/E3 mice at 10 months of age. b-e, Correlations between BODIPY + neutral lipids in DG (% area) and Iba1 + % area ( n = 42) ( b ), AT8 + % area ( n = 31) ( c ), NeuN + % area ( n = 39) ( d ), and hippocampal volume ( n = 39) ( e ) in PS19/E3 mice, assessed via Pearson’s correlation analysis (two-sided). f, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of PS19/E4 mice at 4.0, 6.2, 7.8, and 10.0 months of age. Scale bars, 25μm. g, Correlation between age (in months) and BODIPY + neutral lipids in the hippocampal DG of PS19/E4 mice ( n = 20). Nonlinear regression R 2 . h-j, Representative immunofluorescent images of Rab5 + early endosomes (red, h ), Rab7 + late endosomes (red, i ), and LAMP1 + lysosomes (red, j ) with Iba1 + microglia (blue) and BODIPY + neutral lipid (green) in PS19/E4 mice. All scale bars, 40μm. k, Quantification of BODIPY + marker inside LAMP1 + stain ( n = 15).

    Article Snippet: Coverslips were mounted to microscope slides with VECTASHIELD Prolong Gold with DAPI (H-1200-10, Vectors Labs) or without DAPI if the 405 channel was occupies (P36930, Vector Labs).

    Techniques: Marker, Staining

    a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG mask. Quantified data in b-e represented as mean ± s.e.m., unpaired two-sided t-test. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/NSE-E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm. k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. Quantified data in k and l are represented as mean ± s.e.m., one-way analysis of variance (ANOVA) with Tukey’s post hoc multiple comparisons test. For all experiments, PS19/E4, n = 35; PS19/E3 n = 42; PS19/E4/Syn1-Cre n = 29; and PS19/NSE-E4 n = 18. Pearson’s correlation analysis (two-sided) used for f-i .

    Journal: bioRxiv

    Article Title: Neuronal APOE4 drives damaging lipid accumulation via contact-dependent neuron-oligodendrocyte-microglia interaction in Alzheimer’s disease

    doi: 10.64898/2025.12.04.692390

    Figure Lengend Snippet: a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG mask. Quantified data in b-e represented as mean ± s.e.m., unpaired two-sided t-test. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/NSE-E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm. k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. Quantified data in k and l are represented as mean ± s.e.m., one-way analysis of variance (ANOVA) with Tukey’s post hoc multiple comparisons test. For all experiments, PS19/E4, n = 35; PS19/E3 n = 42; PS19/E4/Syn1-Cre n = 29; and PS19/NSE-E4 n = 18. Pearson’s correlation analysis (two-sided) used for f-i .

    Article Snippet: Coverslips were mounted to microscope slides with VECTASHIELD Prolong Gold with DAPI (H-1200-10, Vectors Labs) or without DAPI if the 405 channel was occupies (P36930, Vector Labs).

    Techniques: